β-elemene inhibits bladder cancer cell through the expression of cell membrane protein GM3

Figure 7 After editing the expression of the GM3 gene, the effect of β-elemene on the expression changes of the GM3-EGFR axis was verified. A: After mRNA detection of GM3 overexpression, intracellular GM3 gene increased to 3-4 times of the original state (^aP < 0.05); B: SiRNA interference reduced intracellular GM3 gene to 1/2 of the original state (^aP < 0.05, ^cP < 0.05, ^eP < 0.05); C and D: After GM3 gene overexpression, intracellular protein expression increased simultaneously, and epidermal growth factor receptor (EGFR) and phosphor EGFR (p-EGFR) expression decreased. The p-EGFR/EGFR ratio did not change; E and F: After GM3 gene expression was inhibited, intracellular protein expression decreased, and EGFR and p-EGFR expression increased. β-elemene can reverse the expression effect of GM3-EGFR/pEGFR. There was no significant difference in EGFR/EGFR ratio.