β-elemene inhibits bladder cancer cell through the expression of cell membrane protein GM3

Figure 2 The CCK8 assay was used to screen the intervention concentration of β-elemene and the effect of β-elemene intervention on the expression of GM3 protein gene and protein in cells. A: CCK8 detection showed that increase in β-elemene concentration induced a gradual decrease in cell activity until the concentration reached 200 μg/mL; further increase in concentration did not induce pronounced changes; B and C: Real-time reverse transcriptase-polymerase chain reaction showed that β-elemene increases GM3 gene expression in TCCSUP and HT-1376 bladder cancer (BLCA) cell lines (^aP < 0.05); D: Immunofluorescence showed that GM3 was localized and expressed in TCCSUP and HT-1376, green for GM3 and blue for DAPI; E and F: Statistics showed that β-elemene increased GM3 expression in TCCSUP and HT-1376 cells (^aP < 0.05); G: Western blotting showed that β-elemene increased GM3 protein expression in TCCSUP and HT-1376 BLCA cell lines; H: Β-elemene increased GM3 protein expression with statistical difference. Western blotting showed that β-elemene increased GM3 protein expression in TCCSUP and HT-1376 BLCA cell lines (^aP < 0.05).