Study of the gut microbiome profile in full-term infants with necrotizing enterocolits

Table 3 Comparison of the gut microbiome between necrotizing enterocolitis patients and controls

Bacteria	NEC	Control	P value	Rank-biserial correlation
Firmicutes			0.492	-0.233
Minimum-maximum	1.89E-01-8.98E-01	1.97E-02-6.01E-01
Median	5.23E-01	4.01E-01
IQR	2.98E-01-6.92E-01	3.12E-01-5.49E-01
Bacteroidetes			0.118	+0.5
Minimum-maximum	1.52E-02-6.84E-01	4.47E-02-7.72E-01
Median	1.10E-01	3.48E-01
IQR	4.19E-02-1.82E-01	2.04E-01-5.45E-01
F/B			0.118	-0.5
Minimum-maximum	0.31-35.79	0.05-6.78
Median	5.42	1.25
IQR	2.31-10.84	0.52-3.25
Prevotella			0.713	+0.133
Minimum-maximum	1.47E-03-1.00E-01	3.06E-04-6.22E-01
Median	6.95E-03	8.33E-02
IQR	4.19E-02-1.82E-01	2.04E-01-5.45E-01
Bacteroides			0.147	+0.467
Minimum-maximum	5.66E-03-6.02E-01	1.64E-02-5.92E-01
Median	4.24E-02	2.17E-01
IQR	3.98E-03-2.86E-02	2.66E-03-2.31E-01
P/B			0.713	+0.133
Minimum-maximum	0.003-0.80	0.001-3.49
Median	0.48	0.51
IQR	0.17-0.64	0.07-1.08
Ruminococcus			0.956	+0.033
Minimum-maximum	0.00E+00-3.41E-02	0.00E+00-6.48E-03
Median	1.11E-03	2.80E-03
IQR	6.38E-06-5.42E-03	6.55E-04-4.43E-03
Lactobacilli			0.875	+0.067
Minimum-maximum	1.34E-02-4.45E-01	1.22E-04-4.87E-01
Median	1.55E-01	2.37E-01
IQR	6.43E-02-2.51E-01	3.79E-02-4.30E-01
Bifidobacteria			0.875	-0.067
Minimum-maximum	8.89E-04-5.58E-01	4.51E-03-1.95E-01
Median	1.24E-01	1.11E-01
IQR	3.92E-03-1.71E-01	9.32E-02-1.24E-01
Akkermancia muciniphila			0.669	-0.117
Minimum-maximum	0.00E+00-8.95E-02	0.00E+00-2.80E-03
Median	0.00E+00	0.00E+00
IQR	0.00E+00-1.17E-05	0.00E+00-0.00E+00
Faecalibacterium prausnitzii			0.492	+0.233
Minimum-maximum	5.37E-04-3.81E-01	0.00E+00-2.54E-01
Median	2.43E-02	8.53E-02
IQR	3.25E-03-3.47E-02	1.50E-02-1.84E-01
Clostridium difficile			-	-
Minimum-maximum	0.00E+00-0.00E+00	0.00E+00-0.00E+00
Median	0.00E+00	0.00E+00
IQR	0.00E+00-0.00E+00	0.00E+00-0.00E+00
Escherichia coli			0.428	-0.267
Minimum-maximum	1.59E-01-8.63E-01	2.15E-02-4.13E-01
Median	3.63E-01	2.84E-01
IQR	2.39E-01-5.90E-01	2.12E-01-3.77E-01
Bacteroides fragilis			0.697	+0.133
Minimum-maximum	0.00E+00-3.00E-01	0.00E+00-3.21E-01
Median	2.43E-03	1.54E-02
IQR	0.00E+00-4.54E-02	2.55E-03-2.63E-02
Lactobacillus reuteri			0.850	+0.05
Minimum-maximum	0.00E+00-1.73E-05	0.00E+00-1.64E-05
Median	0.00E+00	0.00E+00
IQR	0.00E+00-0.00E+00	0.00E+00-0.00E+00
Lactobacillus plantarum			0.947	+0.033
Minimum-maximum	0.00E+00-2.17E-02	0.00E+00-4.81E-03
Median	0.00E+00	0.00E+00
IQR	0.00E+00-2.82E-05	0.00E+00-3.86E-05
Bifidobacterium longum			0.745	-0.117
Minimum-maximum	0.00E+00-2.06E-01	0.00E+00-4.27E-02
Median	1.43E-02	1.64E-02
IQR	3.74E-04-8.09E-02	3.90E-03-2.58E-02
Diversity index			0.092a	+0.533
Minimum-maximum	1.00-1.94	1.60-1.95
mean ± SD	1.529 ± 0.286	1.787 ± 0.147
Median	1.49	1.8
IQR	1.43-1.76	1.68-1.90
Dissimilarity index			-	-
Minimum-maximum	32%-47%	-
mean ± SD	40.100% ± 4.701%	-
Median	40.5%	-

^aP ≤ 0.10 was considered statistically significant.

P value was calculated via the Mann-Whitney test. Rank-biserial correlation: ± 0.00-0.09 (irrelevant), ± 0.10-0.29 (small effect), ± 0.30-0.49 (moderate effect), and ≥ ± 0.50 (large effect)[23]. Negative sign in rank-biserial correlation: Higher rank in the necrotizing enterocolitis group. Positive sign in rank-biserial correlation: Higher rank in control group. NEC: Necrotizing enterocolitis; IQR: Inter quartile range; F/B: Firmicutes/Bacteroidetes; P/B: Prevotella/Bifidobacteria.