Study of the gut microbiome profile in full-term infants with necrotizing enterocolits

Table 1 Primers used in the present study

Target	Primer name	Primer sequence (5’-3’)	Ref.
Lactobacillus plantarum	L. plantarum; FL. plantarum R	ATTCATAGTCTAGTTGGAGGT; CCTGAACTGAGAGAATTTGA	[14]
Lactobacillus reuteri	L. reuteri F; L. reuteri R	GAAGATCAGTCGCAYTGGCCCAA; TCCATTGTGGCCGATCAG	[14]
Bifidobacterium longum	B. longum F; B. longum R	CAG TTG ATC GCA TGG TCT T; TAC CCG TCG AAG CCA C	[14]
Total bacteria	UnivF; UnivR	TCCTACGGGAGGCAGCAGT; GGACTACCAGGGTATCTATCCTGTT	[15]
Akkermansia muciniphila	AM1-F; AM2-R	CAG CAC GTG AAG GTG GGG AC; CCT TGC GGT TGG CTT CAG AT	[16]
Bacteroides	B3F; B3R	CGATGGATAGGGGTTCTGAGAGGA; GCTGGCACGGAGTTAGCCGA	[17]
Bacteroidetes	Bact934F; Bact1060R	GGARCATGTGGTTTATTCGATGAT; AGCTGACGACAACCATGCAG	[17]
Firmicutes	Firm934F; Firm1060R	GGAGYATGTGGTTTAATTCGAAGCA; AGCTGACGACAACCATGCAC	[17]
Bacteroides fragilis	Bact F; Bact R	GAA AGC ATT AAG TAT TCC ACC TG; CGG TGA TTG GTC ACT GAC A	[18]
Bifidobacterium	Bif-F; Bif-R	TCGCGTC(C/T)GGTGTGAAAG; CCACATCCAGC(A/G)TCCAC	[19]
Faecalibacterium prausnitzii	FPR-2F; Fprau645R	GGAGGAAGAAGGTCTTCGG; AATTCCGCCTACCTCTGCACT	[19]
Prevotella	PrevF; PrevR	CACCAAGGCGACGATCA; GGATAACGCCYGGACCT	[17]
Lactobacilli	Lacto-F; Lacto-R	AGCAGTAGGGAATCTTCCA; CACCGCTACACATGGAG	[19]
Ruminococcus	Rflbr730F; Clep866mR	GGCGGCYTRCTGGGCTTT; CCAGGTGGATWACTTATTGTGTTAA	[20]
Clostridioides difficile	C. diff F; C. diff R	TTGAGCGATTTACTT CGGTAAAGA; TGTACTGGCTCACCTTTGATATTCA	[21]
Escherichia coli	E. coli F; E. coli R	CATGCCGCGTGTATGAAGAA; CGGGTAACGTCAATGAGCAAA	[22]