RANKL, a necessary chance for clinical application to osteoporosis and cancer-related bone diseases

Figure 3 Establishment of Ad-sRANKL-injected osteoporosis/hypercalcemia model. Time-dependent changes in serum soluble receptor activator of nuclear factor-κB ligand (sRANKL) (A) and Ca (B) levels. Adenovirus vector harboring mouse sRANKL cDNA (Ad-sRANKL) or Ad-LacZ was injected intraperitoneally into 6-wk-old male C57BL/6 mice at 1.0x10⁹ pfu/mouse in high-dose groups and 3.0 x 10⁸ pfu/mouse in low-dose groups. Serum levels of sRANKL and Ca were measured on day 0, 7 and 14 after injection. Data are presented as the mean ± SD (n = 5 or 6). Ad-sRANKL high dose (filled squares), Ad-sRANKL low dose (filled circles), Ad-LacZ high dose (open squares), Ad-LacZ low dose (open circles). ^aP < 0.05, ^bP < 0.01 vs Ad-LacZ control; C: Micro computer tomography images of femurs in each group of mice. All mice were sacrificed on day 14 after injection and femurs were collected. The upper and lower panels are 3D cross-sectional and longitudinal images, respectively.