Long non-coding RNA GAS5 promotes neuronal apoptosis in spinal cord injury via the miR-21/PTEN axis

Figure 3 Silence growth arrest-specific transcript 5 inhibits neuronal apoptosis in vitro. A: Relative expression levels of miR-21, growth arrest-specific transcript 5 (GAS5), and phosphatase and tensin homolog (PTEN) in PC-12 cells after oxygen-glucose deprivation/reoxygenation treatment with GAS5 knockdown (n = 3 per group); B: Relative mRNA expression levels of caspase-3, B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and AKT with GAS5 knockdown (n = 3 per group); C: Protein expression of cleaved caspase-3, Bcl-2, Bax, PTEN, AKT, and phosphorylated AKT (p-AKT) in PC12 cells with GAS5 knockdown, detected by western blotting (n = 3 per group); D: Immunofluorescence staining for p-AKT and Bax in PC12 cells with GAS5 knockdown; E: TUNEL staining of PC12 cells with GAS5 knockdown. Data are presented as the mean ± SD. ^aP < 0.05, ^bP < 0.01, and ^cP < 0.001 vs control group; ^dP < 0.05, ^eP < 0.01 and ^fP < 0.001 vs oxygen-glucose deprivation/reoxygenation + siCon group.