Long non-coding RNA GAS5 promotes neuronal apoptosis in spinal cord injury via the miR-21/PTEN axis

Figure 2 Growth arrest-specific transcript 5 promoted neuronal apoptosis in the PC12 oxygen-glucose deprivation/reoxygenation model. A: Relative expression levels of microRNA (miR)-21, growth arrest-specific transcript 5, and phosphatase and tensin homolog (PTEN) in PC12 cells subjected to oxygen-glucose deprivation/reoxygenation (OGD/R) treatment, compared to controls (n = 3 per group); B: mRNA relative expression levels of caspase-3, B-cell lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and AKT in PC-12 cells after OGD/R treatment (n = 3 per group); C: Protein expression of cleaved caspase-3, Bcl-2, Bax, PTEN, AKT, and phosphorylated AKT (p-AKT) in PC-12 cells detected by western blotting. The bar graph represents the relative expression levels of the aforementioned proteins. The endogenous control utilized was tubulin (n = 3 per group); D: TUNEL staining of PC12 cells after OGD/R treatment. The data are presented as the mean ± SD. ^aP < 0.05, ^bP < 0.01, and ^cP < 0.001 vs control group.