Altered lineage commitment of bone marrow mesenchymal stem cells in idiopathic osteonecrosis of the femoral head

Figure 3 Adipogenic differentiation of bone-marrow mesenchymal stem cells from osteoarthritis and osteonecrosis of the femoral head patients. Quantification of lipid accumulation was performed by absorbance at 405 nm in osteoarthritis and osteonecrosis of the femoral head mesenchymal stem cell cultures, and light microscopy images of Oil Red O staining after 20 days of induction are shown. Images are representative of 6 independent samples the samples in each group (osteoarthritis, n = 7; osteonecrosis of the femoral head, n = 4). Data represent the mean of the samples analyzed in each group and are shown as mean ± SEM; P values are indicated on the graphs. A: Schematic of mesenchymal stem cell lineage allocation towards adipocytes; B: Expression of adipogenic regulators after induction; relative mRNA levels of peroxisome proliferator-activated receptor gamma and lipoprotein lipase; C: Adipogenic differentiation assessed by Oil Red O staining. OA: Osteoarthritis; ONFH: Osteonecrosis of the femoral head; LPL: Lipoprotein lipase; PPARγ: Peroxisome proliferator-activated receptor gamma; hMSC: Human mesenchymal stem cells. Created with BioRender.com.