Three-dimensional bio-printing: A new frontier in oncology research

Figure 3 Nonsmall-cell lung cancer 95D cell morphology under two-dimensional and three-dimensional culture conditions. The 2D cultured cells (A) are tiled, polygonal, of long spindle shape and display more pseudopodia. In contrast, 3D morphology culture groups (B) are a combination of round and oval shapes, display intercellular tight aggregation and adhesion. Furthermore, there is evidence of multiple sizes of cells distributed in different scaffold pores[60]. 2D: Two-dimensional; 3D: Three-dimensional.