NOTCH1 combined with chemotherapy synergistically inhibits triple-negative breast cancer

Figure 4 Knockdown of NOTCH1 decreases NT5E to inhibit breast cancer proliferation and migration both in vitro and in vivo. A: Western blot results validating the efficiency of stable NOTCH1 knockdown combined with stable NT5E overexpression in MDA-MB-231 cells; B and C: CCK-8 and colony formation assay showing that overexpression of NT5E reversed the inhibitory effect of NOTCH1 knockdown on breast cancer cell proliferation and colony formation; D and E: Transwell and scratch assays demonstrating that NT5E overexpression can reverse the inhibitory effect of NOTCH1 knockdown on breast cancer cell invasion and wound healing; F-H: Mouse xenograft tumor model showing that NT5E overexpression reversed the inhibitory effect of NOTCH1 knockdown on triple-negative breast cancer tumorigenesis in vivo. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001.