NOTCH1 combined with chemotherapy synergistically inhibits triple-negative breast cancer

Figure 1 Knockdown of NOTCH1 inhibits the proliferation and migration of TNBC cells in vitro. A: After viral transduction of MDA-MB-231 cells, successfully transduced cells exhibited green fluorescence under fluorescence microscopy; B and C: RT-PCR and western blotting validation of the knockdown efficiency of NOTCH1 in stable MDA-MB-231 cells; D: CCK-8 assay results show that the proliferation of stable NOTCH1-knockdown MDA-MB-231 cells is reduced compared to wild-type MDA-MB-231 cells; E: Colony formation assay showing that colony formation of stable NOTCH1-knockdown MDA-MB-231 cells was reduced compared to wild-type MDA-MB-231 cells; F: Transwell assay showing that the invasion of stable NOTCH1-knockdown MDA-MB-231 cells was reduced compared to wild-type MDA-MB-231 cells; G: Immunofluorescence showing that after NOTCH1 knockdown, the filamentous arrangement of F-actin in MDA-MB-231 cells was suppressed, and the lamellipodia-like protrusions around the cell periphery were reduced. ^aP < 0.001.