DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods, using four commercially kits

Table 7 Correlation between DNA concentration¹ and clinicopathological aspects of colorectal cancer

Characteristics		P value	MagnaPure LC DNA Isolation kit (magnetic beads)		P value
			< 20 ng/µL	≥ 20 ng/µL
Age	≤ 60	20	12	8	0.383
	> 60	26	12	14
Gender	Male	26	12	14	0.383
	Female	20	12	8
Macroscopic aspect	Polypoid	10	8	2	0.043
	Ulcero-infiltrative	36	16	20
Microscopic aspect	G1	13	4	9	0.023
	G2 + G3	33	23	10
Localization	Proximal colon	8	3	5	0.602
	Distal colon	21	11	10
	Rectum	17	10	7
Depth of infiltration (pT stage)	T2-T3	40	20	20	0.442
	T4	6	4	2
Lymph node metastasis (pN stage)	Present (pN1-3)	17	5	12	0.033
	Absent (pN0)	29	19	10
Lymph node ratio	< 0.15	36	18	18	0.723
	≥ 0.15	10	6	4

¹Automated MagnaPure LC DNA Isolation kit-Roche.

²Chi square test.

³Fisher’s exact test. G1: Well differentiated; G2: Moderately differentiated; G3: Poorly differentiated.