DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods, using four commercially kits

Table 5 Correlation between DNA concentration¹ and clinicopathological aspects of colorectal cancer

Characteristics		Number	iPrep genomic DNA kit (magnetic beads)		P value
			≤ 10 ng/µL	> 10 ng/µL
Age	≤ 60	20	12	8	0.093
	> 60	26	22	4
Gender	Male	26	19	7	0.993
	Female	20	15	5
Macroscopic aspect	Polypoid	10	3	7	0.00043
	Ulcero-infiltrative	36	31	5
Microscopic aspect	G1	13	4	9	< 0.00013
	G2 + G3	33	30	3
Localization	Proximal colon	8	6	2	0.522
	Distal colon	21	17	4
	Rectum	17	11	6
Depth of infiltration (pT stage)	T2-T3 T4	40 6	28 6	12 0	0.112
Lymph node metastasis (pN stage)	Present (pN1-3)	17	14	3	0.313
	Absent (pN0)	29	20	9
Lymph node ratio	< 0.15	36	25	11	0.253
	≥ 0.15	10	9	1

¹Automated iPrep genomic DNA kit-Invitrogen.

²Chi square test.

³Fisher’s exact test. G1: Well differentiated; G2: Moderately differentiated; G3: Poorly differentiated.