DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods, using four commercially kits

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World Journal of Clinical Oncology

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2218-4333

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Observational Study

World J Clin Oncol. Sep 24, 2019; 10(9): 307-317
Published online Sep 24, 2019. doi: 10.5306/wjco.v10.i9.307

Table 2 DNA concentration (ng/µL) using four commercially kits for DNA isolation

	PureLink Genomic DNA mini kit (Invitrogen–manual)	iPrep genomic DNA kit (Invitrogen–automated)	MagnaPure LC DNA Isolation kit (Roche–automated)	High Pure FFPE DNA isolation kit (Roche–manual)
Mean	157.24	8.72	20.39	86.64
Standard deviation	62.99	6.40	21.19	43.84
Minimum	37.60	0.70	0.30	4.20
Maximum	316	29.80	121	168.90

Citation: Kovacs Z, Jung I, Csernak E, Szentirmay Z, Banias L, Rigmanyi G, Gurzu S. DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods, using four commercially kits. World J Clin Oncol 2019; 10(9): 307-317
URL: https://www.wjgnet.com/2218-4333/full/v10/i9/307.htm
DOI: https://dx.doi.org/10.5306/wjco.v10.i9.307

Kovacs Z, Jung I, Csernak E, Szentirmay Z, Banias L, Rigmanyi G, Gurzu S. DNA extraction from paraffin embedded colorectal carcinoma samples: A comparison study of manual vs automated methods, using four commercially kits. World J Clin Oncol 2019; 10(9): 307-317 [PMID: 31572667 DOI: 10.5306/wjco.v10.i9.307]

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