Ethanol consumption as inductor of pancreatitis

Figure 2 Time-course of ethanol-evoked reactive oxygen species (ROS) production in mouse pancreatic acinar cells. Pancreatic acinar cells were loaded with CM-H₂DCFDA, a stable non-fluorescent molecule that yields a polar diol that is well retained within the cells. The diol can then be oxidized by ROS to a fluorescent form; therefore this dye has been proved to be an excellent probe for determination of ROS production. In this setup, stimulation of cells with 50 mmol/L ethanol (EtOH) led to a significant increase in ROS generation. At the end of the experiment H₂O₂ (100 μmol/L) was added, as a positive control for oxidation. a.u., absolute units of fluorescence.