Combination curcumin and vitamin E treatment attenuates diet-induced steatosis in Hfe-/- mice

Figure 3 Altered lipid signalling following high calorie diet feeding is abrogated by curcumin and vitamin E combination therapy. qRT-PCR was used to determine expression levels of hepatic fatty acid oxidation genes in Hfe^-/- mice fed HCD, HCD + Cu, HCD + VE or HCD + Cu + VE. A: Adiponectin receptor 2 (AdipoR2) mRNA; peroxisome proliferator-activated receptor α (PPARα) and carnitine palmitoyl transferase 1A (Cpt1a) mRNA; B: Western blotting and densitometry analysis was performed to determine levels of acyl-coenzymeA oxidase 1 (ACOX) and glyceraldehyde 3-phosphate dehydrogenase (Gapdh). Data are depicted using box and whisker plots showing median, minimum and maximum values. Statistical significance was tested using Kruskal-Wallis test with Dunn’s correction for multiple comparisons, ^aP ≤ 0.05, ^bP ≤ 0.01, ^eP ≤ 0.001. HCD: High calorie diet; Cu: Curcumin; VE: Vitamin E.