P2X7 receptor antagonist recovers ileum myenteric neurons after experimental ulcerative colitis

Figure 7 Double labeling of neuronal nitric oxide synthase and glial fibrillary acidic protein in the rat ileum myenteric plexus in the sham, 2,4,6-trinitrobenzene sulfonic acid and brilliant blue G groups. A-C: Sham group; D-F: 2,4,6-trinitrobenzene group; G-I: Brilliant blue G group. Neuronal nitric oxide synthase immunoreactivity (red; A, D, and G) did not colocalize with glial fibrillary acidic protein immunoreactivity (green; B, E and H). Single arrows indicate labeling of neuronal nitric oxide synthase-positive neurons, and double arrows indicate enteric glial cell positivity. Scale bars = 50 µm. nNOS: Neuronal nitric oxide synthase; GFAP: Glial fibrillary acidic protein; TNBS: 2,4,6-trinitrobenzene sulfonic acid; BBG: Brilliant blue G.