P2X7 receptor antagonist recovers ileum myenteric neurons after experimental ulcerative colitis

Figure 3 Colocalization of the P2X7 receptor with neuronal nitric oxide synthase in neurons of the rat ileum myenteric plexus in the sham, 2,4,6-trinitrobenzene sulfonic acid and brilliant blue G groups. A-C: Sham group; D-F: 2,4,6-trinitrobenzene group; G-I: Brilliant blue G group. Neuronal nitric oxide synthase immunoreactivity (red; A, D, and G) colocalized with P2X7 immunoreactivity (green; B, E and H). Single arrows indicate double-labeled neurons. Scale bars = 50 µm. nNOS: Neuronal nitric oxide synthase; TNBS: 2,4,6-trinitrobenzene sulfonic acid; BBG: Brilliant blue G.