S1PR1 knockdown protects endothelial cells from oxidized low-density lipoprotein-induced injury via reducing interleukin-1β and tumor necrosis factor-α expression

Figure 6 Sphingosine-1-phosphate receptor 1 expression was detected after transfected with siRNA-sphingosine-1-phosphate receptor 1 and treatment with oxidized low-density lipoprotein. A and B: The expression of sphingosine-1-phosphate receptor 1 (S1PR1) was detected by quantitative real-time polymerase chain reaction and western blot after transfected with siRNA-S1PR1 and treatment with 25 μg/mL oxidized low-density lipoprotein (ox-LDL); C: The cell viability was detected by cell counting kit-8 assay after transfected with siRNA-S1PR1 and treatment with 25 μg/mL ox-LDL; D: The apoptosis ratio was detected by flow cytometry after transfected with siRNA-S1PR1 and treatment with 25 μg/mL ox-LDL; E: The interleukin-1β levels were detected by flow cytometry after transfected with siRNA-S1PR1 and treatment with 25μg/mL ox-LDL; F: The tumor necrosis factor-α levels were detected by flow cytometry after transfected with siRNA-S1PR1 and treatment with 25 μg/mL ox-LDL. ^aP < 0.01 vs siRNA-negative control, ^bP < 0.05 vs siRNA-negative control. S1PR1: Sphingosine-1-phosphate receptor 1; ox-LDL: Oxidized low-density lipoprotein; NC: Negative control.