Daming capsule combined with SGLT2i confers protection against diabetes with myocardial ischemia/reperfusion injury induced ferroptosis via AMPK

Figure 5 Ferroptosis induces myocardial damage in vitro model of diabetes mellitus with ischemia-reperfusion. A: Timeline of cell model construction; B: CCK-8 detects cell activity to determine the protective effect of drugs in vitro; C: Representative images of BODIPY 493/503 staining and statistical graph in model groups; D and E: Immunofluorescence was conducted to detect the expression of glutathione peroxidase 4 and 4-hydroxynonenal in model groups; F: Ion-specific fluorescent probe was used to detect the content of iron in model groups; G: Lipid peroxidation sensor BODIPY™ 581/591 C11 was employed to detect lipid ROS levels in model groups; H: The changes of mitochondrial membrane potential in the model groups were analyzed by JC-1 fluorescent probe. Above results are presented as the mean ± SD. The comparison between more groups was conducted by one-way analysis of variance. ^aP < 0.05 vs the control group or the low glucose group; ^bP < 0.05 vs the high glucose + palmitic acid group. LG: Low glucose; HG: High glucose; PA: Palmitic acid; DAPI: 4,6-diamidino-2-phenylindole; CTRL: Control; H/R: Hypoxia/reoxygenation; 4-HNE: 4-hydroxynonenal; GPX4: Glutathione peroxidase 4.