Daming capsule combined with SGLT2i confers protection against diabetes with myocardial ischemia/reperfusion injury induced ferroptosis via AMPK

Figure 4 Sodium-dependent glucose transporters 2 inhibitor combined with daming capsule suppressed ferroptosis in diabetic mice with ischemia-reperfusion. A: Western blot was performed to detect the protein expression of glutathione peroxidase 4 and ferritin heavy chain 1 in mice; B: Iron deposition in cardiac tissue was detected by Prussian blue staining; C: Representative images of BODIPY 493/503 staining of myocardial tissue and statistical graph; D: Transmission electron micrographic image of mitochondrial ultrastructure in mice myocardial tissue. Above results are presented as the mean ± SD. The comparison between more groups was conducted by one-way analysis of variance. ^aP < 0.05 vs the wild type group; ^bP < 0.05 vs the diabetes mellitus + ischemia-reperfusion group or NULL group (diabetes mellitus + ischemia-reperfusion model). WT: Wild type; DMC: Daming capsule; SGLT2i: Sodium-dependent glucose transporters 2 inhibitor; I/R: Ischemia-reperfusion; DM: Diabetes mellitus; GPX4: Glutathione peroxidase 4; FTH1: Ferritin heavy chain 1.