Daming capsule combined with SGLT2i confers protection against diabetes with myocardial ischemia/reperfusion injury induced ferroptosis via AMPK

Figure 1 Diabetes mellitus with ischemia-reperfusion exacerbated cardiac dysfunction. A: Timeline of animal model construction; B and C: Representative images of echocardiogram and early to late diastolic transmitral flow velocity, ejection fraction, fractional shortening, left ventricular internal dimension in diastole and left ventricular internal dimension in systole were analyzed; D: Representative images of hematoxylin and eosin staining, and representative images of masson staining and statistical graph. Above results are presented as the mean ± SD. The comparison between more groups was conducted by one-way analysis of variance. ^aP < 0.05 vs the wild type group; ^bP < 0.05 vs the diabetes mellitus group. WT: Wild type; I/R: Ischemia-reperfusion; DM: Diabetes mellitus; HFD: High fat diet; STZ: Streptozotocin; FBG: Fasting plasma glucose; LVEF: Left ventricular ejection fraction; LVFS: Left ventricular fractional shortening; LVID;s: Left ventricular internal diameter at end-systole; LVID;d: Left ventricular internal diameter at end-diastole; E/A: Early to late diastolic transmitral flow velocity.