Melatonin regulates Sirt1/Nrf2/GPX4 pathway to inhibit ferroptosis and alleviate myocardial injury caused by sepsis

Figure 5 Melatonin activates the sirtuin 1/nuclear factor erythroid 2-related factor 2 axis in vivo and in vitro. A: Representative immunoblots of sirtuin 1, nuclear factor erythroid 2-related factor 2, and heme oxygenase-1 in mouse myocardial tissue (glyceraldehyde-3-phosphate dehydrogenase as control); B: Densitometric quantification normalized to glyceraldehyde-3-phosphate dehydrogenase (in vivo); C: Representative immunoblots of sirtuin 1, nuclear factor erythroid 2-related factor 2, and heme oxygenase-1 in HL-1 cells; D: Densitometric quantification (in vitro). Data are expressed as mean ± SD; in vivo n = 6 mice per group; in vitro n = 3 independent experiments. One-way analysis of variance with Bonferroni post-hoc testing. ^aP < 0.01 vs control; ^bP < 0.01 vs lipopolysaccharide. Ctrl: Control; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; HO-1: Heme oxygenase-1; LPS: Lipopolysaccharide; LPS + Mel: Lipopolysaccharide plus melatonin; LPS + Fer-1: Lipopolysaccharide plus ferrostatin-1; Nrf2: Nuclear factor erythroid 2-related factor 2; Sirt1: Sirtuin 1.