Melatonin regulates Sirt1/Nrf2/GPX4 pathway to inhibit ferroptosis and alleviate myocardial injury caused by sepsis

Figure 2 Histopathology of lipopolysaccharide-induced myocardial injury and protection by melatonin. Representative hematoxylin and eosin-stained myocardial sections from control, lipopolysaccharide (LPS), LPS plus ferrostatin-1, and LPS plus melatonin groups 24 hours after LPS injection. The LPS group shows interstitial edema, hemorrhage/congestion, myofibrillar disruption, and inflammatory cell infiltration; these changes are attenuated in LPS plus ferrostatin-1 and LPS plus melatonin. Representative images from n = 6 mice per group; scale bar = 20 μm. LPS: Lipopolysaccharide; LPS + Mel: Lipopolysaccharide plus melatonin; LPS + Fer-1: Lipopolysaccharide plus ferrostatin-1.