Nicotinamide adenine dinucleotide inhibits the production of reactive oxygen species and myocardial cell pyroptosis caused by hypoxia/re-oxygenation injury

Figure 5 Western blot analysis of the protein levels of NOD-like receptor pyrin domain-containing 3, associated speck-like protein containing a CARD, and procaspase-1 in H9c2 cells after hypoxia/re-oxygenation treatment, with or without acetylcysteine or nicotinamide adenine dinucleotide oxidized form (NAD⁺) (n = 3). NLRP3: NOD-like receptor pyrin domain-containing 3; ASC: Associated speck-like protein containing a CARD; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; H/R: Hypoxia/re-oxygenation; NAD: Nicotinamide adenine dinucleotide; NAD⁺: Nicotinamide adenine dinucleotide oxidized form.