NBCe1 Na+-HCO3- cotransporter ablation causes reduced apoptosis following cardiac ischemia-reperfusion injury in vivo

Figure 4 TUNEL staining to quantify apoptosis in WT and KO heart sections after ischemia and reperfusion. Mice were subjected to 30 min of ischemia by occlusion of the LAD, followed by 3 h of reperfusion, and heart sections were processed to analyze apoptosis. A: Representative images from WT and KO heart sections, taken from a region approximately 1 mm distal to the occlusion site, showing nuclei stained with DAPI (blue), TUNEL (red) and sarcomeric actin (green). Yellow arrows point to apoptotic nuclei (pink); B: Quantification of apoptotic nuclei. n = 4 mice of each genotype, P ≤ 0.05.