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World J Biol Chem. May 26, 2013; 4(2): 30-34
Published online May 26, 2013. doi: 10.4331/wjbc.v4.i2.30
Published online May 26, 2013. doi: 10.4331/wjbc.v4.i2.30
Figure 3 Adenosine triphosphate/nigericin-induced caspase-1 activation and externalization in human monocytes are not blocked by human α-defensin 5.
Lipopolysaccharide (LPS)-activated monocytes were stimulated with Adenosine triphosphate (ATP, 1 mmol/L) or nigericin (Nig) (30 μmol/L) in the presence or absence of human α-defensin 5 (HD-5) (50 μg/mL) for 1.5 h. Media were harvested, precipitated, and subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot analysis with anti-caspase-1 p10 antibodies (1:1000) that recognize both the pro-caspase-1 (45 kDa) and cleaved caspase-1 p10 subunit proteins.
- Citation: Galliher-Beckley AJ, Lan LQ, Aono S, Wang L, Shi J. Caspase-1 activation and mature interleukin-1β release are uncoupled events in monocytes. World J Biol Chem 2013; 4(2): 30-34
- URL: https://www.wjgnet.com/1949-8454/full/v4/i2/30.htm
- DOI: https://dx.doi.org/10.4331/wjbc.v4.i2.30