Caspase-1 activation and mature interleukin-1β release are uncoupled events in monocytes

Figure 2 Human α-defensin 5 blocks interleukin-1β release from monocytes stimulated with Adenosine triphosphate or nigericin. Overnight-cultured, lipopolysaccharide (LPS)-activated, 35S-Met/cys-labeled human monocytes were treated with Adenosine triphosphate (1 mmol/L) or nigericin (Nig) (30 μmol/L) in the presence or absence of human α-defensin 5 (HD-5) (50 μg/mL) for 1.5 h. Media and cell-associated fractions were harvested separately. Interleukin-1β (IL-1β) was recovered from each by immunoprecipitation and resulting immunoprecipitates were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and autoradiography. Images are representative from three independent experiments.