Localization and function of a eukaryotic-initiation-factor-2-associated 67-kDa glycoprotein

Figure 6 Levels of p67 and deglycosylated p67 in normal breast and breast cancer cells. Normal established breast cell line (MCF-10, lane 1) and three primary breast cancer cell lines (lanes 2-4) were lysed in NP-40 lysis buffer. A: The levels of p67 and deglycosylated p67 (DG-p67) in the total lysates were detected by Western blotting analysis; B: Semi-quantitative analysis of the levels of p67 and DG-p67 using ImageJ (v 1.42k, NIH). The levels of p67 and DG-p67 were normalized with the level of p67 in MCF10 cells.