Mitochondrial alanyl-tRNA synthetase 2 mediates histone lactylation to promote ferroptosis in intestinal ischemia-reperfusion injury

Figure 3 Mitochondrial alanyl-tRNA synthetase 2 epigenetically upregulates acyl-CoA synthetase long-chain family member 4 expression via histone lactylation. A and B: The (A) protein expression [Protein levels of acyl-CoA synthetase long-chain family member 4 (ACSL4) in Caco-2 cells normalized to β-actin. Bar graphs show densitometric quantification (mean ± SD)] and (B) RNA level of ACSL4 in Caco-2 cells that depleted of AARS2; C: The enrichment of H3K18 La in histone of ACSL4 gene; D: The luciferase activity of ACSL4 gene promoter. ^aP < 0.01 vs shNC.