Basic Study
Copyright ©The Author(s) 2025.
World J Gastrointest Surg. Jun 27, 2025; 17(6): 106777
Published online Jun 27, 2025. doi: 10.4240/wjgs.v17.i6.106777
Figure 2
Figure 2 Mitochondrial alanyl-tRNA synthetase 2 increases in vitro cell injury. A: Protein level of mitochondrial alanyl-tRNA synthetase 2 (AARS2) in cells. Protein levels of AARS2 in cells normalized to β-actin. Bar graphs show densitometric quantification (mean ± SD); B: Cell viability was detected by CCK-8; C: mRNA abundance of ZO-1 and occluding; D: Levels of Fe2+ and malondialdehyde; E: Protein levels of GPX4 and acyl-CoA synthetase long-chain family member 4 (ACSL4) in cells. Protein levels of GPX4 and ACSL4 in cells normalized to β-actin. Bar graphs show densitometric quantification (mean ± SD). aP < 0.01 vs cont, bP < 0.01 vs hypoxia-reperfusion. H/R: Hypoxia-reperfusion; AARS2: Mitochondrial alanyl-tRNA synthetase 2.