Mitochondrial alanyl-tRNA synthetase 2 mediates histone lactylation to promote ferroptosis in intestinal ischemia-reperfusion injury

Figure 1 Mitochondrial alanyl-tRNA synthetase 2 promotes intestinal ischemia-reperfusion injury and ferroptosis in mouse model. A: Protein level of mitochondrial alanyl-tRNA synthetase 2 (AARS2) in intestinal tissue. Protein levels of AARS2 in intestinal tissues normalized to β-actin. Bar graphs show densitometric quantification (mean ± SD); B: Hematoxylin and eosin staining of intestinal tissues; C: Serum level of Fatty acid-binding protein measured by ELISA; D: mRNA abundance of ZO-1 and occluding; E: Levels of Fe²⁺ and malondialdehyde; F: Protein levels of GPX4 and acyl-CoA synthetase long-chain family member 4 in intestinal tissues; G: Production of tumor necrosis factor-alpha and interleukin-6 measured by ELISA. ^aP < 0.01 vs sham, ^bP < 0.01 vs model. AARS2: Mitochondrial alanyl-tRNA synthetase 2.