Mechanism of the epidermal growth factor receptor in promoting endothelial cell dysfunction in gestational diabetes mellitus

Figure 5 EIF4A3 combined with long noncoding RNAs H19 increases the expression of epidermal growth factor receptor to promote high glucose-induced human umbilical vein endothelial cells dysfunction. A: Epidermal growth factor receptor protein expression detected by Western blotting; B: Cell viability detected by cell counting kit-8; C: Cell apoptosis detected by flow cytometry; D: Cell migration assessed by scratch experiments; E: Cell migration assessed by Transwell assays; F: Tube formation assay to assess angiogenesis in cells; G: Western blot showing the expression of endothelial cell dysfunction markers. ^aP < 0.05. ^bP < 0.01. ^cP < 0.001. EGFR: Epidermal growth factor receptor; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; NC: Normal control; HG: High glucose; OE-H19: Overexpression H19; sh-EIF4A3: Short hairpin RNA targeting EIF4A3; PI: Propidium iodide; FITC: Fluorescein isothiocyanate; VCAM-1: Vascular cell adhesion molecule-1; TNF-α: Tumor necrosis factor-α; VEGF-A: Vascular endothelial growth factor-A; ICAM-1: Intercellular cell adhesion molecule-1.