Mechanism of the epidermal growth factor receptor in promoting endothelial cell dysfunction in gestational diabetes mellitus

Figure 3 Epidermal growth factor receptor expression is regulated by long noncoding RNAs H19. A: H19 expression in human placental tissue detected via quantitative real-time polymerase chain reaction (RT-qPCR); B: H19 expression in cells detected via RT-qPCR; C: H19 expression in mouse placental tissue detected via RT-qPCR; D: H19 expression in cells detected via RT-qPCR; E: EGFR messenger RNA expression in cells detected via RT-qPCR; F: EGFR protein expression in cells detected via Western blotting. ^bP < 0.01. ^cP < 0.001. GDM: Gestational diabetes mellitus; NC: Normal control; HG: High glucose; sh-NC: Short hairpin-negative control; sh-H19: Short hairpin RNA targeting H19; OE-EGFR: Overexpression epidermal growth factor receptor; EGFR: Epidermal growth factor receptor; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.