Mechanism of the epidermal growth factor receptor in promoting endothelial cell dysfunction in gestational diabetes mellitus

Figure 1 Epidermal growth factor receptor is differentially expressed in gestational diabetes mellitus. A: Epidermal growth factor receptor (EGFR) messenger RNA (mRNA) expression in human placental tissue detected by quantitative real-time polymerase chain reaction (RT-qPCR); B: Colocalization of EGFR and cluster of differentiation 31 detected by immunofluorescence double staining; C: EGFR mRNA expression in cells detected by RT-qPCR; D: The expression of the EGFR protein was detected by Western blotting; E: RT-qPCR detection of EGFR mRNA expression in mouse placental tissue; F: Western blot detection of EGFR protein expression in the mouse placenta. ^bP < 0.01. ^cP < 0.001. mRNA: Messenger RNA; EGFR: Epidermal growth factor receptor; CD31: Cluster of differentiation 31; GDM: Gestational diabetes mellitus; DAPI: 4’,6-diamidino-2-phenylindole; NC: Normal control; HG: High glucose; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.