Ras homolog enriched in brain 1 regulates β cell mass and β cell function via mTORC1/AMPK/Notch1 pathways

Figure 6 Rheb1 regulates transcription factor HNF4α. A: Protein expression level of HNF4α was increased in Rheb1 overexpressed MIN6 cells (n = 3); B: Protein expression level of HNF4α was decreased in Rheb1 knockout MIN6 cells; C: The interaction between Rheb1 and HNF4α were analyzed by co-IP in MIN6 cells; D: After overexpression Rheb1 in human islets for 48 hours, immunofluorescence staining of Rheb1 and HNF4α in human islets. All data are represented as the mean ± SD, ^aP < 0.05, ^bP < 0.01. NC: Negative control.