Ras homolog enriched in brain 1 regulates β cell mass and β cell function via mTORC1/AMPK/Notch1 pathways

Figure 3 Rheb1 promotes β cell proliferation only partly depend on the mTORC1. A: The population of 5-ethy-nyl-2’-deoxyuridine + cells of MIN6 cells treated with PWPI or Rheb1OE lentivirus with or without rapamycin treatment; B: The population of Ki67+ cells of insulin+ islet treated with GFP or Rheb1OE adenovirus with or without rapamycin treatment; C: Western blot analysis of C57 mice islets treated with GFP or Rheb1OE adenovirus for 48 hours with or without rapamycin treatment (n = 3/group). All data are represented as the mean ± SD, ^aP < 0.05, ^bP < 0.001. NC: Negative control; Edu: 5-ethy-nyl-2’-deoxyuridine.