Ras homolog enriched in brain 1 regulates β cell mass and β cell function via mTORC1/AMPK/Notch1 pathways

Figure 2 Rheb1 activates mTORC1 signaling in islets. A: The expression of mTORC1 signaling and its downstream in the islets isolated from pRheb1KO (n = 2) and Flox (n = 2) with or without glucose stimulation; B: The expression of mTOC1 signaling and its downstream in the islets isolated from pRheb1KO (n = 3) and Flox (n = 3) with or without glucose and insulin stimulation; C: Western blot analysis of C57 islets treated with GFP (n = 3) or Rheb1OE (n = 3) adenovirus for 48 hours with or without glucose and insulin stimulation. All data are represented as the mean ± SD, ^aP < 0.05, ^bP < 0.01. NC: Negative control.