RNA-binding protein CPSF6 regulates IBSP to affect pyroptosis in gastric cancer

Table 1 Correlation between integrin binding sialoprotein expression and clinicopathologic characteristics in gastric cancer patients

Parameter	n	Low IBSP expression (n = 8)	High IBSP expression (n = 22)	P value
Age (yr)
≥ 60	11	4	7	0.361
< 60	19	4	15
Tumor diameter (cm)
≥ 3	10	6	4	0.004a
< 3	20	2	18
Gender
Male	14	3	11	0.544
Female	16	5	11
TNM stage
I + II	7	5	2	0.002a
III + IV	23	3	20
Distant metastasis
M0	15	5	10	0.409
M1	15	3	12

^aP < 0.05 was considered to have a significant difference.

Categorical variables were compared by the chi-square test. IBSP: Integrin binding sialoprotein.

Table 2 Cleavage and polyadenylation factor 6 regulates integrin binding sialoprotein to aggravate gastric cancer progression

No.	Findings in previous studies	Findings in this work
1	IBSP has been discovered to exhibit higher expression and important regulatory function in colorectal cancer, breast cancer, and esophageal squamous cell carcinoma. However, the regulatory functions of IBSP in GC progression remain unclear	IBSP exhibits higher expression in GC tissues and cell lines. IBSP facilitates GC cell proliferation, migration, and invasion but suppresses pyroptosis
2	Previous studies have verified the oncological function of the RNA-binding protein CPSF6 in acute myeloid leukemia, breast cancer, and GC. However, no reports have focused on the regulatory effects of CPSF6 on metastasis and pyroptosis	CPSF6 promotes cell proliferation, migration, and invasion but boosts pyroptosis
3	The RNA-binding protein CPSF6 binds to the 3’-UTR of genes to participate in the progression of hepatocellular carcinoma, lung adenocarcinoma, and GC. But, the relationship between IBSP and CPSF6 has not been studied in GC progression	CPSF6 binds to the 3’-UTR of IBSP and positively regulates IBSP expression
4	The regulatory mechanism (RNA binding protein-mRNA 3’-UTR) exists in GC progression. However, the regulatory effects of CPSF6/IBSP remain unclear	The retarded GC progression mediated by CPSF6 knockdown is reversed by IBSP overexpression

IBSP: Integrin binding sialoprotein; CPSF6: Cleavage and polyadenylation factor 6; 3’-UTR: 3’-untranslated region; GC: Gastric cancer.