EPZ020411, a PRMT6 inhibitor, suppresses colorectal cancer cell proliferation, migration, and invasion through targeting the TGM2/MAPK axis

Figure 4 Transglutaminase 2 overexpression enhances colorectal cancer cell proliferation. A: Circular plasmid map of lentiviral vector pLV3-CMV-TGM2 (human)-5-Puro; B and C: Western blot was used to detect the expression levels of transglutaminase 2 protein levels in HCT116 (B) and CACO-2 (C) cells; D and E: Representative image of colony formation assays of HCT116 (D) and CACO-2 (E) cells; F and G: EdU staining was performed to detect the proliferation of HCT116 (F) and CACO-2 (G) cells (scale bar: 50 μm). Error bars represented the mean ± SEM. ^aP < 0.01, ^bP < 0.001. TGM2: Transglutaminase 2.