Targeting cyclin-dependent kinase 6 partially reverses drug resistance in ripretinib-resistant gastrointestinal stromal tumor cells in vitro

Figure 7 Cyclin-dependent kinase 6 expression correlates with protein kinase B expression and activation. A: Protein levels in gastrointestinal stromal tumor cell lines, including cyclin-dependent kinase 6 (CDK6), protein kinase B (AKT) and phosphorylated-AKT; B: MRNA levels in gastrointestinal stromal tumor cell lines, including CDK6, AKT; C: In the ripretinib-resistant cell lines, both the protein and mRNA levels of CDK6 were significantly decreased in the group treated with the AKT inhibitor; D: Effects of CDK6 inhibitors on the expression of apoptosis-related proteins in ripretinib-resistant cells. ^aP < 0.05, ^bP < 0.001. CDK6: Cyclin-dependent kinase 6; AKT: Protein kinase B; p: Phosphorylated; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; Ri: Ripretinib; P: Palbociclib; RI: Resistance index; BAX: B-cell lymphoma-2-associated X protein; Bcl-2: B-cell lymphoma-2.