Dietary eicosapentaenoic and docosahexaenoic acids decrease prostanoids in the ileum of rats treated with irinotecan and 5-fluorouracil

Table 4 Cytokines and chemokine in ileum tissue of rats treated with chemotherapy and fed eicosapentaenoic acid + docosahexaenoic acid diet, mean ± SEM

Immune signals (pg/mg of protein)	Baseline	Control diet			EPA + DHA diet			Main effects		Interaction
	Day 0 (n = 5)	Day 2 (n = 4)	Day 4 (n = 4)	Day 8 (n = 4)	Day 2 (n = 5)	Day 4 (n = 4)	Day 8 (n = 4)	P value (diet)	P value (day)	P-diet × day
TNF-α	74 ± 2.4	58 ± 6.61	60 ± 6.6	93 ± 1.6	62 ± 7.11	69 ± 2.8	100 ± 6.9		< 0.001
IL-10	88 ± 3.0	67 ± 3.6	74 ± 15	110 ± 18	77 ± 8.6	87 ± 10	110 ± 21
Fractalkine	710 ± 31	600 ± 311	930 ± 120	890 ± 76	610 ± 501	946 ± 68	860 ± 79		< 0.001
IL-1β	220 ± 28	110 ± 151	140 ± 22	300 ± 35	140 ± 301	146 ± 23	330 ± 69		< 0.001
IL-6	480 ± 7.6	480 ± 9.8	490 ± 3.7	500 ± 9.5	500 ± 5.7	490 ± 6.7	520 ± 9.4

¹Denotes significant differences between days on the same diet (time effect).

EPA: Eicosapentaenoic acid; DHA: Docosahexaenoic acid; IL: Interleukin; TNF: Tumor necrosis factor.