Dietary eicosapentaenoic and docosahexaenoic acids decrease prostanoids in the ileum of rats treated with irinotecan and 5-fluorouracil

Table 1 Total phospholipid fatty acids in ileum tissue of rats treated with chemotherapy and fed eicosapentaenoic acid + docosahexaenoic acid diet, mean ± SEM

Phospholipid fatty acids (% w/w)	Baseline	Control diet			EPA + DHA diet			Main effects		Interaction
	Day 0 (n = 5)	Day 2 (n = 4)	Day 4 (n = 4)	Day 8 (n = 4)	Day 2 (n = 5)	Day 4 (n = 4)	Day 8 (n = 4)	P value (diet)	P value (day)	P value (diet and day)
18: 2n-6	3.9 ± 0.38	3.8 ± 0.163	5.3 ± 0.17	4.0 ± 0.19	3.9 ± 0.31	5.0 ± 0.17	4.4 ± 0.22		0.0003
20: 4n-6	9.7 ± 0.28	11 ± 0.251,3	8.9 ± 0.47	10 ± 0.16	10 ± 0.133	8.0 ± 0.30	8.6 ± 0.332	0.001	< 0.001	0.031
Total n-6	14 ± 0.39	15 ± 0.16	14 ± 0.17	14 ± 0.20	14 ± 0.33	13 ± 0.20	13 ± 0.24	0.049	0.003
18: 3n-3	0.024 ± 0.009	0.002 ± Tr3	0.053 ± Tr	0.050 ± 0.011	0.027 ± 0.0203	0.057 ± 0.005	0.058 ± 0.014		0.002
20: 5n-3	Tr	0	0.002 ± 0.001	Tr	0.058 ± 0.0353	0.54 ± 0.102	0.80 ± 0.122	< 0.001	< 0.001	< 0.001
22: 6n-3	0.29 ± 0.01	0.27 ± 0.005	0.31 ± 0.003	0.30 ± 0.02	0.31 ± 0.0143	0.52 ± 0.032	0.69 ± 0.022	< 0.001	< 0.001	< 0.001
Total n-3	0.31 ± 0.018	0.27 ± 0.005	0.37 ± 0.005	0.36 ± 0.027	0.40 ± 0.0693	1.1 ± 0.142	1.5 ± 0.152	< 0.001	< 0.001	< 0.001

¹Denotes significant differences between baseline and day 2 in rats fed the control diet (chemotherapy effect).

²Denotes significant differences between diets on the same day (diet effect).

³Denotes significant differences between days within the same diet (time effect).

Tr: Trace; EPA: Eicosapentaenoic acid; DHA: Docosahexaenoic acid.