IQGAP1 promotes tumor progression by stabilizing MCM3/Nrf2 in cholangiocarcinoma

Figure 3 IQGAP1 recruited USP28 to stabilize MCM3 expression. A: Silver-stained plots of the IQGAP1 immunoprecipitated proteins, in which MCM3 interacted with IQGAP1; B: Co-IP consequence confirmed that IQGAP1 significantly precipitated MCM3 and USP28 in two cholangiocarcinoma (CCA) cell lines; C: After knocking down or overexpressing IQGAP1, MCM3 showed the same trend of change as IQGAP1, while USP28 protein levels remained unchanged; D: Overexpression of IQGAP1 led to an increase in MCM3 that interacted with USP28, while knockdown of IQGAP1 reduced the interaction between USP28 and MCM3; E: Immunofluorescence results showed that MCM3 and IQGAP1 were co-localized in CCA cells. IgG: Immunoglobulin G; NC: Negative control; OE: Overexpression; si: Silence; IP: Immunoprecipitation.