ASAP3 disrupts ASAP1-ARHGAP12 to inhibit RhoA and yes-associated protein/transcriptional coactivator with PDZ-binding motif, suppressing gastric cancer progression

Figure 2 Effects of ASAP3 on the growth of gastric cancer cells. A: Cell cycle of HGC-27 and AGS cells after transfection with short hairpin (sh)-ASAP3 or over-expression (oe)-ASAP3 plasmids using a flow cytometry; B: Apoptosis of HGC-27 and AGS cells after transfection with sh-ASAP3 or oe-ASAP3 plasmids using a flow cytometry; C: Migration of HGC-27 and AGS cells after transfection with sh-ASAP3 or oe-ASAP3 plasmids by cell scratch assay; D: Migration of HGC-27 and AGS cells after transfection with sh-ASAP3 or oe-ASAP3 plasmids by Transwell assay; E: Invasion of HGC-27 and AGS cells after transfection with sh-ASAP3 or oe-ASAP3 plasmids by Transwell assay; F: The colony-forming ability of HGC-27 and AGS cells after transfection with sh-ASAP3 or oe-ASAP3 plasmids using clone formation assay. ^cP < 0.05 vs short hairpin-negative control-HGC-27 cell. ^dP < 0.05 vs over-expression-negative control-AGS cell. oe: Over-expression; sh: Short hairpin; NC: Negative control.