ASAP3 disrupts ASAP1-ARHGAP12 to inhibit RhoA and yes-associated protein/transcriptional coactivator with PDZ-binding motif, suppressing gastric cancer progression

Figure 1 ASAP3 expression in gastric cancer, and cell transfection efficiency. A: Relative expression of ASAP3 in gastric cancer (GC) cell lines (AGS and HGC-27 cells); B: Cell transfection efficiency assessed by determining the messenger RNA (mRNA) expression of ASAP3 in AGS transfected with over-expression-ASAP3 plasmids; C: Cell transfection efficiency assessed by determining the mRNA expression of ASAP3 in HGC-27 transfected with short hairpin-ASAP3 plasmids; D: Proliferation of GC cells (HGC-27 and AGS) after transfection using cell counting kit-8 after cultured for 24 hours, 48 hours, and 72 hours. ^aP < 0.05 vs AGS cell. ^bP < 0.05 vs HGC-27 cell. ^cP < 0.05 vs short hairpin-negative control-HGC-27 cell. ^dP < 0.05 vs over-expression-negative control-AGS cell. mRNA: Messenger RNA; oe: Over-expression; sh: Short hairpin; NC: Negative control.