TCHP regulates the proliferation and apoptosis of gastric cancer cells via AKT/BCL2 signaling pathway

Figure 4 TCHP knockdown modulates the expression of cell cycle- and apoptosis-related proteins and affects associated signaling pathways. A: Western blotting shows that TCHP knockdown upregulates p21 and downregulates cyclin D1 and CDK4. This pattern indicates the induction of G1 phase cell cycle arrest; B: Western blotting of AKT signaling and apoptosis-related proteins shows that TCHP knockdown reduces p-AKT and BCL2 expression, while increasing BAX levels. These changes suggest an inhibition of AKT-mediated survival signaling and promotion of apoptosis. Data are presented as mean ± SD from three independent experiments. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. NC: Negative control.