TCHP regulates the proliferation and apoptosis of gastric cancer cells via AKT/BCL2 signaling pathway

Figure 1 TCHP is upregulated in gastric cancer and promotes tumor cell proliferation. A: Protein expression levels of TCHP in the gastric mucosal epithelial cell line (GES-1) and two gastric cancer (GC) cell lines (AGS and HGC-27) were detected by Western blotting; B: TCHP expression was significantly higher in GC tissues compared with adjacent non-tumor tissues; C: High TCHP expression was significantly associated with a lower overall survival rate in patients with GC; D: Western blotting confirmed the knockdown efficiency of TCHP in AGS and HGC-27 cells transfected with shTCHP3, compared to the negative control group; E: Cell proliferation following TCHP knockdown was assessed in AGS and HGC-27 cells using CCK-8 assay; F: Cell proliferation following TCHP knockdown was assessed in AGS and HGC-27 cells using colony formation assay. Data are presented as mean ± SD of three independent experiments. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. NC: Negative control; STAD: Stomach adenocarcinoma; TCGA: The Cancer Genome Atlas.