IGF2BP1 promotes gastric cancer progression by stabilizing HS6ST2 mRNA in an N6-methyladenosine-dependent manner

Figure 4 Heparan sulfate 6-O-sulfotransferase 2 promotes the proliferation, migration, and invasion of gastric cancer cells in vitro. A and B: Quantitative reverse-transcription polymerase chain reaction (A) and western blot (B) for the knockdown efficiency of heparan sulfate 6-O-sulfotransferase 2 (HS6ST2) in SGC7901 cells at the mRNA and protein levels; C and D: Quantitative reverse-transcription polymerase chain reaction (C) and western blot (D) analyses of HS6ST2 overexpression efficiency in MGC803 cells at the mRNA and protein levels; E and F: Transwell (E) and Wound healing assay (F) for the effect of HS6ST2 knockdown on the invasion and migration ability of gastric cancer (GC) cells; G and H: Transwell (G) and Wound healing assay (H) for the effect of overexpression on the migration ability of GC cells; I and J: Cell counting kit-8 assay for the effect of HS6ST2 knockdown (I) and overexpression (J) on the proliferation of GC cells. Data are expressed as mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. HS6ST2: Heparan sulfate 6-O-sulfotransferase 2; shRNA: Short hairpin RNA; GAPDH: Gyceraldehyde-3-phosphate dehydrogenase; NC: Negative control; OE: Overexpression.