IGF2BP1 promotes gastric cancer progression by stabilizing HS6ST2 mRNA in an N6-methyladenosine-dependent manner

Figure 2 Knockdown of insulin-like growth factor 2 mRNA-binding protein 1 inhibits the proliferation, migration, and invasion of gastric cancer cells in vitro. A and B: Quantitative reverse-transcription polymerase chain reaction analysis of insulin-like growth factor 2 mRNA-binding protein 1 (IGF2BP1) knockdown efficiency at the mRNA level in MGC803 (A) and SGC-7901 (B) cells; C and D: Western blotting confirming IGF2BP1 knockdown at the protein level in MGC803 (C) and SGC-7901 (D) cells; E: Transwell assays showing the effects of IGF2BP1 knockdown on the migration and invasion abilities of MGC803 and SGC-7901 cells; F: Wound healing assays assessing the effects of IGF2BP1 knockdown on the migration ability of MGC803 and SGC-7901 cells; G: Cell counting kit-8 assay evaluating the effects of IGF2BP1 knockdown on cell proliferation in MGC803 and SGC-7901 cells. Data are presented as mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. IGF2BP: Insulin-like growth factor 2 mRNA-binding protein; shRNA: Short hairpin RNA; NC: Negative control; GADPH: Gyceraldehyde-3-phosphate dehydrogenase; CCK-8: Cell counting kit-8.